JHOR Journal of Hematalogy and Oncology Research 2372-6601 Open Access Pub United States JHOR-20-3189 10.14302/issn.2372-6601.jhor-20-3189 research-article RETRACTED: Assessment of the Risk of Hemochromatosis in Polytransfused Sickle Cell Patients at the Abidjan Transfusion Therapy Unit Yassongui Mamadou Sekongo 1 * Kabore Saydou 2 Kouakou Boidy 3 Kouamenan Sidonie 4 Kassogue Kadidja 5 N’Guessan Parfait 5 Oue Nabo Bertin 5 Konate Seidou 6 Sanogo Ibrahima 7 Department of Training and Research, National Blood Transfusion Center; Abidjan; Côte D’Ivoire Laboratory of quality control, National Blood Transfusion Center; Abidjan; Côte D’Ivoire Hematology Department, UFR Medical Sciences; Abidjan; Côte D’Ivoire Hemovigilance Service, National Blood Transfusion Center; Abidjan; Côte D’Ivoire Transfusion Therapy Unit, National Blood Transfusion Center; Abidjan; Côte D’Ivoire Director of the NBTC, National Blood Transfusion Center; Abidjan; Côte D’Ivoire Hematology Service, Teaching Hospital Center of Yopogon, Abidjan; Côte D’Ivoire Corresponding Author Li-Pin Kao 1 Research Professor, East Carolina University, U.S.A. Sekongo Yassongui Mamadou, Unité de Recherche et de Thérapeutique Transfusionnelle, Centre National de Transfusion Sanguine, Abidjan, Côte d’Ivoire, BP V15 km 4 Bd de Marseille, E-mail: sekyass@yahoo.fr

The authors have declared that no competing interests exist.

 01 04 2020 3 4 7 13 24 01 2020 26 03 2020 01 04 2020 © 2020 Yassongui Mamadou Sekongo, et al This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. This article is available from http://openaccesspub.org//jhor/article/1306

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In Côte d'Ivoire, sickle cell disease affects 14% of the population. It is responsible for significant morbidity and mortality. Transfusion is a significant element in the management of major sickle cell anemia, which exposes them to post-transfusion hemochromatosis. The biological diagnosis is based on the determination of serum iron and the transferrin saturation coefficient (CST). As the determination of the CST was not available in our exercise context in Côte d'Ivoire, we determined only the ferritinemia. The interest of this work lies in the therapeutic implication linked to the identification of patients at risk of hemochromatosis because chelators are difficult to access for most patients. This was a prospective, descriptive and analytical study, on polytransfused sickle cell patients, followed at the transfusion therapy unit (UTT) of the CNTS of Abidjan, from 2010 to 2018. We included 78 sickle cell patients, all ages and genders who have received at least ten transfusions. The ferritinemia assay was carried out by ELISA. Transfusion exchange, with 59% of cases, was the most used mode of transfusion. The mean ferritinemia was 1719.19 ng / ml. Hyperferritinemia was found in 63% of patients. Most of the patients were on a long-term transfusion program with an average of 27.5 bags of red blood cell concentrates. Thirty-two patients had received at least 20 bags of red blood cell concentrates. We noted 21 patients treated, including 3 with deferoxamine and 18 treated with oral deferasirox. We have identified 33 sickle cell anemia patients at risk for hemochromatosis. The determinants of the risk of hemochromatosis were the high number of blood bags and the method of transfusion.

 hemochromatosis Sickle cell desease tranfusion Introduction

The complications of sickle cell disease require transfusion therapy in its severe forms. People whose treatment involves iterative transfusions are exposed to frequent complications including hemochromatosis or hemosiderosis.

Hemochromatosis is the body's iron overload.

There are two types of hemochromatosis: the genetic form and the secondary forms. These secondary forms are linked to iterative transfusions 1234.

The biological diagnosis of post transfusion hemochromatosis is based on the determination of ferritinemia and the determination of the transferrin saturation coefficient (CST) 5. The diagnosis of post-transfusion hemosiderosis is suspected in the case of ferritinemia greater than 1000 ng / mL, measured after the transfusion of at least 20 bags of red blood cell concentrates 67

Post transfusion hemochromatosis is not documented in Côte d'Ivoire. Very few doctors research it to their polytransfused patients. In France, although notification of post-transfusion hemosiderosis has been compulsory since 1994 in the French haemovigilance network, it has so far been largely ignored despite the obligation to report cases 8

We are interested in the identification of patients at risk of hemochromatosis by measuring only ferritinemia

The objective of our work was to contribute to the description of the development of sickle cell anemia in Côte d'Ivoire.

 Methodologie

It was a retrospective, prospective, descriptive and analytical study on polytransfused sickle cell patients followed in the transfusion therapy unit (UTT) of the National Blood Transfusion Center of Abidjan in Côte d’Ivoire, from 2010 to 2018. The study concerned patients collected over a period of 8 years. The work lasted 5 months from May to September 2018.

Our Study Population concerned 78 major sickle cell anemia patients, of all ages and of all sexes, admitted to this unit and having benefited from at least ten (10) transfusions.

We proceeded by simple random sampling of consenting polytransfused sickle cell patients followed at the UTT.

We included polytransfused sickle cell patients followed at UTT who received at least ten transfusions and who benefited from the ferritinemia test during our study period. We excluded all patients with an infectious and / or inflammatory syndrome and those with a sickle cell vaso-occlusive crisis.

The biological material used for the study consisted mainly of venous blood taken from a dry tube after the informed consent of each patient.

The ferritinaemia assay of patients was carried out by the ELISA technique (Enzyme-Linked- Immuno-Sorbent- Assay) according to the manufacturer's recommendations on a “Mini-Vidas”

The data collected was entered using EXCEL 2013 software.

The study was carried out using software EPI.INFO version:

The statistical analysis of the data and the preparation of tables and figures was done using EPI INFO software version 7.2.2.6 and excell 2010.

 Results Epidemiologicals aspects

(Table 1).

Distribution according epidemiologicals aspects
PARAMETERS   NUMBER PERCENTAGE
Phénotype Hémoglobinique SSFA2 50 64,1
SFA2 26 33,3
SC 1 1,3
SAFA2 1 1,3
  78 100
Age group ˂ 15 26 33
15 - 30 41 53
31 - 45 8 10
46 - 60 3 4
TOTAL 78 100
Sex Femal 38 49
Male 40 51
TOTAL 78 100
Sex-ratio: 1,05  
Biological and Therapeutic Aspects

Distribution of Patients by Ferritin Level

(Figure 1).

Distribution by ferritin level Transfusion Mode

(Table 2).

Patients distribution according to the method of transfusion.
TRANSFUSION MODE NUMBER PERCENTAGE(%)
Simple Transfusion 32 41
Exchange Transfusion 39 50
Exchange and Simple Transfusion 07 9
TOTAL 78 100

The half of the sickle cell patients (39) in our study had exchange transfusion i.e. 50%, versus 32 patients for simple transfusion, i.e. 41%

Number Red Cells Pockets Transfused

(Table 3).

Distribution of patients according to the number of red cells bags transfused
NUMBER OF RED CELLS BAGS TRANSFUSES NUMBER PERCENTAGE(%)
10 à 20 30 38,46
21 à 30 32 41,03
31 à 40 10 12,82
41 à 50 01 1,28
51 à 60 02 2,56
61 and plus 03 3,85
TOTAL 78 100
Treatment of Hemochromatosis Whether or not Treatment

(Figure 2).

Distribution of patients at risk of hemochromatosis according to the existence or no treatment The Type of Chelating Treatment

(Table 4).

Distribution of treatment according to the type of iron chelator
TYPE OF CHELATING NUMBER PERCENTAGE
 DEFEROXAMINE   03  14,29
 DEFERIPRONE 00 00
  DEFERASIROX  18  85,71
 TOTAL 21 100
Evolution of Post Transfusion Hemochromatosis

(Table 5).

Distribution of patients according to evolution
    HEALED  DEAD  TOTAL
 TREATED  21  00  21
 UNTREATED  00  06  12
 TOTAL  21  06  27
Discussion

In our study, the average age of our sickle cell patients was 21 years. The age group between 15 and 30 years predominated with 41 patients ((53%). We found 26 (33%) patients under 15 years of age while, 11 (14%) patients were between 30 and 60 years old. This shows that the majority of sickle cell patients are young. Our work corroborates those carried out in Côte d'Ivoire and and other counties of Africa. ELIRA D and al 9 in Congo reported an average age of 21.8 ± 3.7 with extremes of 2 and 45 years.

Our study included adults, proving that the sickle cell major lives beyond 15 years 89 The improvement in the life expectancy of sickle cell patients in Africa has been widely documented by Sangare and al 10. This is explained by protocols for the early management of sickle cell anemia from birth, including the use of antianemia, vasodilators, non-steroidal anti-inflammatory drugs 1112.

The sex-ratio in our study was 1.05 with 51% for men and 49% for women. There is therefore no tendency towards any sexual predisposition of the affection.

The anemic forms of sickle cell anemia (SSFA2 and SFA2) represented 64.10% and 33.34% respectively, so they are the most exposed to complications. Our results are similar to those of Sekongo and Coll. 13 who obtained for the phenotypes SSFA2, SFA2, SAFA2 and SC respectively 57.24%, 25.52%, 2.07% and 15.17%. This predominance of anemic forms in our study population is logical since they were patients selected on the base of polytransfusion.

The mean of ferritinemia was 1,719.19 ng / ml. This ferritinemia ranged from 55ng / ml to 8800ng / ml with 63% hyperferritinemia (49 patients) and 37% normal ferritinemia (27 patients). Our results are superior to those of Raouf Hafsia et al. 14 in Tunisia who found in their study an average ferritinemia at 660.35 ng / ml with extremes of 4.89 and 3360 ng / ml) in their sickle cell patients.

Out of a Total of 49 Hyperferritinemic Patients

-16 patients (33%) had a ferritinemia between 200 or 300 and 1000ng / ml,

-33patients (67%) had ferritinemia above 1000ng / ml who presented with hemochromatosis. Our results are superior to those of Raouf Hafsia and al. 14 who found ferritinemia levels greater than 1000ng / ml in only 23.41% of their polytransfused sickle cell patients.

The transfusion exchange with 50% was the most used mode. The patients, in general, were in transfusion program because they had complications such as stroke, priapism, leg ulcer or severe forms of vaso –occlusive crisis, pregnancy, osteonecrosis.

For simple transfusions, the majority of patients did so as part of ad hoc therapy with severe anemia as an indication.

For normal ferritinaemia, the majority of patients had benefited from the transfusion exchange. The transfusion exchange had an influence on the ferritinemia despite the fact that it was mechanical thus obliging to bleed before transfusing. Today the transfusion therapy unit has within it an "OPTIA" type erythrapheresis device. According to De Montalembert M., 2 the practice of erythrapheresis (transfusion exchange using a cell separating machine) seems to avoid this serious disadvantage

The majority of our patients were on a long-term transfusion program, hence the increase in the number of transfusions with an average of 27.5 red blood cells and the risk of iron overload.

Among 33 hyperferritinemic patients at risk of hemochromatosis, 21 (61%) were treated and 12 (36%) untreated

Regarding management, 32 out of 33 patients at risk for hemochromatosis had received at least 20 (97%) red blood cells. This result corroborates that of the committee of experts of the Limonsin hematology network which states: "from 20 globular pellets with ferritinemia at 1000ng/ml, it is absolutely necessary to chelate” 15. In our study, one patient (3) escaped this committee rule, which should be notified. The finding made at the UTT of the Abidjan CNTS has been proven. In this unit it would therefore be recommended to make the chelation from 10 transfusions with a ferritinemia above 1000ng / ml.

In our study, the determinants of hemochromatosis risk were the number and the mode of transfusions.

The average of ferritinemia in 33 patients at risk of hemochromatosis was 4680.86ng/ml with the ferritin level between 1066 ng/ml and 9540ng/ml (p = 0.0000).

The average number of Red blood Cell received by patients at risk for hemochromatosis was 35 bags with extremities from 17 to 74 bags (p = 0.0001).

Our results corroborate those of Fung EB and Coll 16 as well as Harmatz P et al. 17 who all reported in their studies a significant correlation between increased ferritinemia and the number of red blood cells transfused in sickle cell patients. Davies and al 18, by evaluating ferritinemia in 31 homozygous sickle cell patients, showed that it was significantly higher in polytransfused patients

Out of a total of 33 hyperferritinemic patients at risk of hemochromatosis, 26 (79%) patients practiced simple transfusion and 01 (3) patient for transfusion exchange (p = 0.00000).

For 33 hyperferritinemic patients at risk of hemochromatosis, 21 (64%) were treated and 12 (36%) untreated. Among the patients treated, 03 (14,29%) had used deferoxamine as a subcutaneous or intravenous injection for at least two months and 18 (85.71%) patients or were treated with deferasirox for at least two months by the oral route. Deferasirox also offers an alternative to subcutaneous infusions for children from 2 years of age. Treatment with deferasirox met with patient support and markedly increased adherence to chelating therapy. The same observations have been made by Brittenham GM 19 and Clara Camaschella 20.

The patients who had been treated with iron chelation were all cured of iron overload. For those who could not be treated either because of the cost of treatment or the shortage of the drug, or because of late detection, half were deceased Hemochromatosis in sickle cell anemia is a factor of mortality.

 Conclusion

At the end of our study, we identified 33 polytransfused sickle cell patients at risk of hemochromatosis. The determinants of the risk of hemochromatosis were the number and the mode of transfusions.

Our study highlights the need to inform patients about certain aspects of their management, to ask and carefully measure the indication for transfusion in the event of even marked anemia. It would be recommended to start chelation beyond 20 administered red blood cells bags, as soon as the ferritinemia is above 1000ng/ml. The practice of transfusion exchanges, automated or manual, will limit the risk of iron overload. However, the cost of ferritin dosing must be within everyone's reach and considered as a routine exam in polytransfused sickle cell anemia. Chelators must be accessible and available in pharmacies.

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